Helicase Product Solutions

Full-length Recombinant Active Helicases for Screening, Structural Analysis, and Mechanism of Action Studies

Helicases are promising therapeutic targets for various disease states, such as cancer and microbial infections, that warrant in-depth investigation. Helicase enzymes are molecular motors that use the energy of ATP to unwind DNA or RNA duplexes. They play critical roles in almost every aspect of nucleic acid metabolism, including replication, recombination, repair, transcription, and telomere maintenance.

Eurofins DiscoverX® provides functionally active recombinant helicase enzymes specifically designed for drug discovery applications. These full-length helicases have been validated for substrate-dependent activity via ATP to ADP conversion (using the ADP Hunter Kit) and DNA/RNA strand displacement assays. Biophysical testing was performed using surface plasmon resonance (SPR) and Microscale Thermophoresis (MST) to confirm the suitability of the helicases for ligand binding and mechanism of action (MOA) studies. These enzymes are also suitable for cryo-electron microscopy (cryo-EM) evaluation for high-resolution structure analysis to further accelerate structure-based drug design.

Discover how helicase enzymes can propel your research—from initial screening to lead optimization—and drive the next generation of innovative therapeutics.

Product Highlights
  • Highly Pure Recombinant Enzymes – High purity helicases optimized for biochemical and biophysical screening, including high-throughput screening (HTS) campaigns
  • Full-length, Functionally Validated Proteins – Thoroughly confirmed for activity using ADP accumulation and DNA/RNA strand displacement assays
  • Validated – Enzymes validated through Eurofins Discovery Helicase screening services
  • Broad Assay Compatibility – Support for a variety of MOA studies, including ligand binding assays, SPR and MST analysis, and high-resolution structural analysis by cryo-EM

Consider Eurofins DiscoverX’s custom development capabilities for custom cell lines, assays, & enzyme development.

Products

  • Helicases - Explore recombinant helicases expressed in bacterial and insect systems, designed for DNA and RNA unwinding studies, protein-DNA/RNA interactions, and pathways such as replication, repair, and transcription
  • ADP Accumulation Assays - Antibody-free, homogeneous, and gain-of-signal activity-based assays for identifying and characterizing kinase phosphotransferase activity. Learn more about ADP Accumulation Assays.