[2016 Biopharmaceutical Symposium] Rapidly Develop Simple Potency Assays for Interleukins & Their Receptors
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- 2016 Biopharmaceutical Symposium Poster
Ligand-induced receptor dimerization is an early functional step in receptor activation, representing the most proximal, functional read-out for Interleukin receptors. It is well understood that the family of Interleukin receptors will dimerize with the other members of its family leading to a complicated signaling cascade that is critically involved in a variety of auto-immune, inflammatory and oncogenic diseases. Here we present a novel application of the PathHunter® technology to monitor receptor-receptor interactions at the surface of intact live cells, applicable to diverse receptor types, with a specific focus on the interleukin family of receptors. The high specificity, simplicity of the assay protocol, large signal to noise ratio, serum tolerance and reproducibility of these assays has enabled their use in cell-based screening, functional characterization and QC lot release assays. Examples discussed here include assays for ~80% of all human interleukins, including assays for Ustekinumab and Tocilizumab. These robust assays have excellent reproducibility, accuracy and precision, demonstrating their suitability for use as QC Lot Release assays.