Functional Receptor Tyrosine Kinase Assays for Antibody therapeutics Using PathHunter® Technology

Version:: 0310_REV1
Year:: 2010

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Kinase targets have been extensively studied in biochemical assays using purified protein fragments for the kinase and the substrate. However, there is an increasing need to understand how kinases function in the context of a whole cell assay. DiscoveRx has pioneered a protein interaction detection system using well-established Enzyme Fragment Complementation (EFC) technology, by monitoring the interaction of target proteins with SH2 phosphotyrosine binding domains. We have successfully applied this approach to multiple receptor tyrosine families. Over the recent past, monoclonal antibodies have become a growing class of potential drugs for cancer and other therapeutic areas. In this study, we demonstrate the application of the PathHunter cell-based kinase assays to detect anti-receptor and anti-ligand antibody that function specifically against EGFR, c-Met, TrkA. This data demonstrates that PathHunter™ Cell-Based kinase assays provide a robust, sensitive and easy-to-use method for identifying novel antibodies in a whole cell, homogenous assay that is also high-throughput friendly.