PathHunter® Cell-Based Kinase Assays: A Portfolio of HTS-friendly Cytoplasmic or Receptor Tyrosine Kinase Assays for Small Molecule Inhibitors as well as Monoclonal Antibodies

PathHunter® Cell-Based Kinase Assays: A Portfolio of HTS-friendly Cytoplasmic or Receptor Tyrosine Kinase Assays for Small Molecule Inhibitors as well as Monoclonal Antibodies
Version:
SBSP_0410

File Name/Number:
SBS 2010

Year:
2010

Kinase therapeutics includes both monoclonal antibodies (mAbs) and small-molecule inhibitors. DiscoveRx’s PathHunter® protein: protein interaction system uses well-established Enzyme Fragment Complementation (EFC) technology by monitoring interaction of target proteins with SH2 phosphotyrosine binding domains. We demonstrate application of PathHunter® cell-based kinase assays in detecting anti-receptor/anti-ligand antibody and small molecule inhibitors for EGFR, c-Met, CSF3R and Trk A. Results suggest that these assays provide a robust, sensitive and one-step cell-based assay for identifying novel antibodies and small molecule inhibitors.