[User Manual] KILR® Assay for Suspension Cell Lines
- File Name/Number:
The KILR cytotoxicity cell line enables the development of a simple, non-radioactive and dye-free method to specifically measure target cell death in a co-culture. The KILR assay has several applications in iOnc drug development including measuring target cell death through Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), Antibody Dependent Cell Phagocytosis (ADCP), Cytotoxic T Cell Lymphocyte-Mediated Death (CTL), Bi-specific Antibody-Mediated T Cell Redirection, Chimeric Antigen Receptor T Cell (CAR-T) and adoptive T cell therapies. This assay can drive all aspects of the drug discovery process, from screening to characterization, and the development of a QC lot release assay for the immunotherapy drugs.
Each KILR Assay target clonal cell line or stable pool has been characterized for its stability in culture, and has been found to be stable for at least 15 passages. Assays should be run using fresh, low-passage cells that are seeded and rapidly followed by opsonization with antigen-specific antibodies where appropriate.