[User Manual] PathHunter® β-Arrestin eXpress GPCR Assay
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PathHunter β-Arrestin cell lines are stable clonal cell lines that expedite drug discovery and development by providing robust response to over 90% of all known G-protein coupled receptor (GPCRs), with accurate pharmacology. This assay measures an essential pathway in GPCR activation, i.e., β-arrestin recruitment to activated GPCRs, enabling scientists to screen for and profile functional agonists and inhibitors of GPCRs. These assays are successfully used to identify and optimize biologics and small molecule drugs. Since β-arrestin recruitment occurs independent of G-protein coupling, these assays provide a direct, universal platform for measuring receptor activation. The PathHunter eXpress GPCR Assay can be used for small or large molecules. The kits provide a robust, highly sensitive and easy-to-use assay for monitoring G-protein coupled receptor (GPCR) activity by detecting the interaction of β-arrestin with the activated GPCR.