[User Manual] PathHunter® eXpress Activated GPCR Internalization Assay
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PathHunter® eXpress Activated GPCR Internalization Assays are ready to use complete kits that contain everything you need to measure arrestin bound internalized GPCRs in live cells without the hassle of using antibodies, radioactivity or elaborate imaging. The eXpress kits include single use vials of frozen cells stably expressing the GPCR of interest, optimized cell plating reagent, chemiluminescent detection reagents and plates. Simply thaw and plate the pre-validated cells and challenge with compound 24 or 48 hours later. Whether you are studying receptor recycling, identifying functional antagonists, or determining mechanism of action of your lead compounds, the ready-to-assay eXpress format eliminates the need for lengthy, expensive and time consuming cell culture and makes functional testing fast and convenient. Assays are designed for 96-well plate analyses and kits include enough cells and detection reagents for either 100, 200 or 1000 datapoints.