[User Manual] PathHunter® GPCR Explorer Kit
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PathHunter® GPCR Explorer kits are a simple and easy-to-use method for building on-demand functional GPCR assays. Whether you have a panel of mutant GPCR cDNAs, a favorite ortholog GPCR or a GPCR heterodimer you’d like to functionally test, the simple, optimized transfection protocol combined with PathHunter EFC based chemiluminescent detection makes it the ideal platform for exploring novel GPCR receptor biology. The Explorer kits are configured in an ultra-convenient, assay-ready complete kit format that includes frozen, transfection-ready CHO-K1 cells, optimized detection reagents, cell plating reagent and assay plates. Assays are designed for 96-well plate analysis and kits include enough cells and detection reagents for either 100 or 1000 data points.