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Receptor Dimerization Assays

Quantitative assays for receptor dimer formation

Ligand-induced receptor dimerization is the first step following receptor activation and the most proximal and reliable indicator of receptor activation. Until now, these interactions have not been successfully monitored using classic cellular protein interaction techniques. The PathHunter® Dimerization assay platform enables you to measure these interactions on a cell surface and is ideal for lead identification, quality control and development of neutralizing antibody (Nab) assays for a variety of biologics, including bi-specific antibodies. View Validation Data »
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Assay Benefits & Highlights

  • Functional target specific response
  • Simple one-step, plate-treat-read protocol
  • Validated for the development of biologics
  • Flexible - use for screening, potency and Nab assays

Dimerization Assay Principle

The two target receptors are tagged with ProLink™ (PK) or Enzyme Acceptor (EA). Upon ligand-induced activation, the receptors dimerize forcing the two β-gal components to complement and create an active enzyme. Active β-gal generates a chemiluminescent signal in the presence of substrate.

Screen & optimize peptides, antibodies or even small molecules

Profile & optimize bi-specific antibodies Create cell-based assays for heterodimer pairs based on native receptor biology


Cytokine AssayCell LineLigandPart No.eXpress Kit
ACVR1B/BMPR2 U2OS Activin A 93-1072C3 93-1072E3CP5S
ACVR1C/ACVR2 U2OS Activin B 93-1069C3 93-1069E3CP5S
ACVR1C/ACVR2B U2OS Activin B 93-1047C3 93-1047E3CP5S
ACVR2/ACVR1 U2OS BMP-7 93-0906C3
ACVRL1/ACVR2 U2OS GDF-2 (BMP-9) 93-0963C3
ACVRL1/ACVR2B U2OS GDF-2 (BMP-9) 93-0964C3 93-0964E3CP22S
ACVRL1/BMPR2 U2OS GDF-2 (BMP-9) 93-0962C3 93-0962E3CP22S
BMPR1A/ACVR2 U2OS BMP-4 93-1037C3 93-1037E3CP5S
BMPR1A/ACVR2B U2OS BMP-4 93-1038C3
BMPR1A/BMPR2 U2OS BMP-4 93-1006C3 93-1006E3CP0S
BMPR1B/ACVR2 U2OS BMP-6 93-1010C3 93-1010E3CP0S
BMPR1B/ACVR2B U2OS BMP-6 93-1052C3
BMPR1B/BMPR2 U2OS BMP-6 93-1053C3
c-MET/c-MET U2OS HGF 93-1063C3
CSF1R/CSF1R U2OS M-CSF 93-1061C3 93-1061E3CP5S
CSF2RB/CSF2RA U2OS GM-CSF 93-1078C3 93-1078E3CP22S
EGFR/EGFR U2OS EGF 93-0989C3 93-0989E3CP5S
EGFR/ErbB2 U2OS EGF 93-1051C3 93-1051E3CP14S
EpoR/EpoR U2OS Erythropoietin 93-0965C3 93-0965E3CP0S
ErbB2/ErbB3 U2OS Heregulin-β2 93-1042C3
ErbB2/ErbB4 U2OS Heregulin-β1 93-0960C3 93-0960E3CP5S
ErbB4/ErbB4 U2OS Heregulin-β2 93-0961C3 93-0961E3CP5S
FGFR3(G380R)/FGFR3(G380R) U2OS FGF-18 93-1091C3
FGFR3/FGFR3 U2OS FGF-18 93-1055C3
IFNAR1/IFNAR2 HEK 293 IFN-beta 93-1097C1
IFNGR1/IFNGR2 U2OS IFN-γ 93-1098C3 93-1098E3CP5S
IFNRL1/IL10RB U2OS IFN-λ1 (IL-29) 93-0993C3 93-0993E3CP5S
IL10RA/IL10RB U2OS IL-10 93-0985C3 93-0985E3CP0S
IL11RA/IL6ST HEK 293 IL-11 93-1028C1
IL12RB1/IL12RB2 U2OS IL-12 93-1041C3 93-1041E3CP0S
IL17RA/IL17RC U2OS IL17A 93-0999C3 93-0999E3CP0S
IL17RA/IL17RC HEK 293 IL17A 93-0931C1 93-0931E1CP7S
IL17RD/TNFR2 U2OS TNFα (human) 93-1068C3 93-1068E3CP5S
IL18R1/IL18RAP U2OS IL-18 93-1044C3 93-1044E3CP5S
IL1R1/IL1RAP U2OS IL-1α 93-1032C3 93-1032E3CP5S
IL1RL1/IL1RAP U2OS IL-33 93-1067C3 93-1067E3CP5S
IL20RA/IL10RB U2OS IL-26 93-1020C3 93-1020E3CP0S
IL20RA/IL20RB U2OS IL-24 93-1027C3 93-1027E3CP0S
IL21R/IL2RG U2OS IL-21 93-1035C3 93-1035E3CP0S
IL22RA1/IL10RB HEK 293 IL-22 93-1026C1 93-1026E1CP7S
IL23R/IL12RB1 U2OS IL-23 93-1007C3 93-1007E3CP0S
IL2RB/IL2RG U2OS IL-2 93-0998C3 93-0998E3CP5S
IL2RB/IL2RG/IL2RA U2OS IL-2 93-1003C3 93-1003E3CP0S
IL31RA/OSMRb U2OS IL-31 93-1002C3 93-1002E3CP5S
IL3RA/CD131 HEK 293 IL-3 93-0969C1
IL4R/IL13RA1 U2OS IL-13 93-1000C3 93-1000E3CP5S
IL4R/IL13RA1 HEK 293 IL-13 93-1001C1 93-1001E1CP0S
IL4R/IL2RG U2OS IL-4 93-0988C3 93-0988E3CP5S
IL5RA/CD131 U2OS IL-5 93-0972C3 93-0972E3CP0S
IL6R/IL6ST U2OS IL-6 93-1045C3 93-1045E3CP5S
IL7R/CRLF2 DLD1 TSLP 93-1019C13 93-1019E13CP25S
IL7R/IL2RG DLD1 IL-7 93-0997C13 93-0997E13CP0S
IL9R/IL2RG HEK 293 IL-9 93-1036C1
KDR/KDR HEK 293 VEGF-121 93-0996C1 93-0996E1CP0S
LEPR/LEPR U2OS Leptin 93-1107C3
OSMR/IL6ST U2OS Oncostatin M 93-1056C3 93-1056E3CP5S
TGFBR1/ACVR2 U2OS GDF-11 93-1012C3 93-1012E3CP5S
TGFBR1/ACVR2B U2OS GDF-11 93-1046C3 93-1046E3CP5S
TGFBR1/TGFBR2 U2OS TGF-β1 93-0889C3 93-0889E3CP5S
TGFBR1/TGFBR2 U2OS TGF-β1 93-0889C3 93-0889E3CP5S
TIM3/TIM3 U2OS Anti-TIM3 Antibody [344801] 93-1082C3 93-1082E3CP5S
TPOR/TPOR HEK 293 93-1108C1
VISTA/VISTA U2OS Human VISTA Antibody 93-1079C3 93-1079E3CP5S