Detection Reagents
DiscoverX offers detection reagents with both chemiluminescent and fluorescent readouts to suit the diverse needs of your drug discovery programs. DiscoverX’s chemiluminescent detection is based on the proprietary Enzyme Fragment Complementation (EFC) technology, while the fluorescent detection is based on an activated fluorescent dyes. These detection reagents are optimized for a variety of applications such as detecting cell-based protein-protein interactions, compound-protein binding, translocation, and second messenger assays.
DiscoverX’s detection reagents are designed for DiscoverX cell lines including cAMP Hunter™, InCELL Hunter™ or PathHunter® cell lines, InCELL assays, and ProLabel®/ProLink™ (PL/PK) expression or cloning vectors inside whole cells.
Product Highlights
- Sensitive detection and reproducible results providing robust assays you can rely on
- Easy, one-step addition protocols ideal for multiple basic research and drug discovery applications
- Scalable from 96- to 3456-well formats compatible with high-throughput screening or miniaturization
Reagent Calculator Cell Lines
Chemiluminescent Detection Products
Detection Kits for Functional Confirmation of EFC Component Expression
| Product |
Cat No. |
Intended Use |
| EFC BlocDetect™ Kit |
92-0004 |
Detect false positive results by identifying compounds that inhibit β-galactosidase complementation or β-galactosidase activity |
| ProLink Detection Kit |
92-0006 Series |
Detect ProLink-tagged protein in parental cells expressing ProLink Vectors |
| PathHunter ProLabel Detection Kit |
93-0180 Series |
Detect ProLabel-tagged protein in parental cells expressing ProLabel Vectors |
| PathHunter EA Detection Kit |
93-0183 Series |
Detect large protein fragment of β-galactosidase (Enzyme Acceptor, EA) in parental cells expressing EA such as ENDO-EA, MEM-EA, NUC-EA, β-arrestin EA, and SH2-EA |
| PathHunter PL/PK Detection Kit |
93-0812 Series |
For functional confirmation of EFC components with PathHunter Pathway cell lines
|
Fluorescence Detection Products
| Product |
Cat No. |
Intended Use |
| HitHunter Calcium No WashPLUS Assay |
90-0091 Series |
Monitor GPCR and ion channel activation using Native Calcium Gq-Coupled Cell Lines |
| Coelenterazine h |
90-0084 |
| ADP Hunter™ Plus |
90-0083 Series |
Detect kinase activity by monitoring ATP depletion |
| ADP Quest™ |
90-0071 Series |
Kinase and inhibitor characterization for kinetic or endpoint analysis |