Epigenetic Proteins Product Solutions

Epigenetics involves the study of heritable or acquired changes in gene expression caused by non-genetic mechanisms without alterations in gene structure or sequence. Basic epigenetic mechanisms such as histone modifications, DNA methylation, and chromatin remodeling are essential in the regulation of many physiological processes. Alterations to the mechanisms -- either by modifications to DNA and histone components of nucleosomes, or by expression of noncoding RNAs (ncRNAs) -- can have implications in a number of cancers, immunological, and neurodegenerative conditions.

Eurofins DiscoverX® offers epigenetics recombinant proteins and InCELL Hunter™ cell-based assays for studying the modulation of epigenetic mechanisms and identifying small molecule inhibitors and modifiers of DNA methyltransferases and bromodomains. Utilize InCELL Hunter cell-based compound-target engagement assays to confirm small molecule compound cell entry and epigenetic target binding, screen and rank inhibitors, validate hits identified in biochemical assays, and measure cellular EC50 values all in the native cellular environment.

Product Highlights
  • Biologically Relevant - Obtain quantitative cellular compound entry and epigenetic target binding data for more confident drug discovery decisions
  • Easy-to-Use & Scalable - Simple, homogeneous, and high-throughput amenable protocol to easily measure compound binding to your epigenetic target with a chemiluminescent readout
  • Target Specificity & Selectivity - Ability to screen and differentiate multiple target-selective stereoisomers of BRD4 and determine target specificity
  • High Quality Proteins - Access epigenetics-related recombinant proteins produced using optimal expression systems with high quality and purity

Consider Eurofins DiscoverX’s custom development capabilities for custom cell lines, assays, & enzyme development.

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Products

  • Epigenetic Proteins – Recombinant epigenetic proteins, expressed in E. coli and tagged (e.g. GST, His6), and used in EPIscan screening and profiling services
  • InCELL Hunter Epigenetic Cell Lines – Stable cell lines confirmed for past 10 passages and rigorously and continuously validated for accurate pharmacology through Eurofins Discovery services with billions of data points screened.
  • InCELL Hunter eXpress Kits – Complete assay-ready kits with specific cell line, optimized reagents, and plates for quick Enzyme Fragment Complementation (EFC) based chemiluminescent detection.
  • InCELL Pulse Starter Kit – Create your own cell-based, target engagement assays with reagents and protocol for the design and development of InCELL Pulse assays for the measurement of inhibitor cellular potency against user-defined epigenetic targets of interest.
  • InCELL Detection Kit – Measure compound binding to a specific protein target in InCELL Hunter and InCELL Pulse cell lines