Receptor Tyrosine Kinase Assays

Cell-based Assays to Analyze RTK Dimerization, Phosphorylation, Internalization, and SH2-recruitment

Receptor tyrosine kinases (RTKs) play important roles in a variety of cellular processes, including growth, motility, differentiation, and metabolism. Recent large-scale genomic studies have revealed the presence of various alterations in the genes encoding RTKs, such as EGFR, HER2/ErbB2, and MET, among many others. Dysregulation of RTK signaling leads to an assortment of human diseases, most notably numerous cancers. Abnormal RTK activation in human cancers is mediated by four principal mechanisms: gain-of-function mutations, genomic amplification, chromosomal rearrangements, and/or autocrine activation.

Eurofins DiscoverX® offers PathHunter® cell-based assays for RTK and cytokine receptors associated with cytosolic tyrosine kinases (CTK). These innovative assays provide cellular context to the activation of these receptors, as well as for the identification of novel inhibitors and therapeutic antibodies. Utilize the PathHunter assays to analyze RTK and cytokine receptor functional activity by analyzing their dimerization, phosphorylation, internalization, and SH2-recruitment.

Product Highlights
  • Broadly Applicable - Identify various ligands, including anti-receptor, anti-ligand, or activating antibodies; non-ATP pocket binders (allosteric modulators); ligand binding inhibitors (ATP-competitors); or dimerization inhibitors
  • High Specificity - Tagged, tyrosine kinase eliminates background from endogenous tyrosine kinases
  • Biologically Relevant - MOA-reflective, functional assays for monitoring RTK and cytokine receptor activation and dimerization for characterization through screening applications

Consider Eurofins DiscoverX’s custom development capabilities for custom cell lines, assays, & enzyme development

SHOP PRODUCTSKINASE TARGET LIST

Products

Eurofins DiscoverX offers a variety of cell-based assays in the form of cell lines and ready-to-use kits for functional activity, dimerization, SH2 recruitment, characterization, and screening of RTKs and CTKs associated with cytokine receptors.

  • RTK Assays - Functional and activity assays to analyze RTK dimerization, phosphorylation, internalization, and SH2-recruitment of RTKs.
  • RTK Bioassay Kits - Qualified and target-based bioassays for evaluating RTK and cytokine receptor dimerization, SH2-recruitment, and IκB degradation, including CSF2, EGF, FGF, GH, IGF1, Insulin Receptor, Interleukin, ErbB2 (via HER2), PDGF, TrkA/B/C, and VEGF receptors including KDR. Learn more about Bioassay Kits.
  • RTK eXpress Assay Kits - Ready-to-use cell-based assay kits for quick analysis and implementation in early discovery phases for screening applications, proof-of-concept, mechanism of action confirmation, and rank-ordering studies. Learn more about eXpress Assay Kits.
  • RTK Cell Lines - Stable cell lines developed to reflect the target’s mechanism of action with a drug interaction in a standard cell line background. Learn more about Stable Cell Lines.