Protein Translocation

Track Cellular Movement of Proteins to Sub-cellular Compartments

The movement of proteins from the plasma membrane to endosomes, endoplasmic reticulum to the plasma membrane, or cytoplasm to the nucleus is essential for the protein's specific biological role. When natural movements are altered by therapeutic binding, protein mutations, or abnormal signaling, undesirable consequences such as drug tolerance, unwanted side effects, therapeutic toxicity, and diseases may occur. Measuring therapeutic binding that affects translocating proteins provides insight into these adverse effects and ultimately helps to identify safer drugs.

Eurofins DiscoverX® PathHunter® protein translocation assays provide the ability to study the internalization and cellular trafficking of your protein of interest to and from different cellular compartments and analyze the pharmacological effects of therapeutics on these events. Unlike alternative methods that require specialized equipment, antibodies, or fluorescent tags, these cell-based assays are high-throughput, easy-to-use, quantitative, and highly specific and sensitive.

Product Highlights
  • Simple, Homogeneous Assay – Explore protein translocation to multiple cellular compartments using an easy-to-use, non-imaging, high throughput cell-based assay platform
  • Pharmacochaperone Discovery – Identify small molecule compounds that functionally rescue disease relevant mutant proteins involved in cystic fibrosis, Alzheimer’s disease, Huntington’s disease, and others
  • Biologics Discovery – Quantify peptide and antibody-based therapeutics targeting receptors that can induce high levels of translocation
  • Flexible Solutions – Create quantitative, custom cell-based assays to study the translocation of any protein (e.g., GPCRs, RTK, checkpoint receptors, nuclear proteins, ion channels, & more)

Consider Eurofins DiscoverX’s custom development capabilities for custom cell lines, assays, & enzyme development.

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GPCR Internalization Assays
GPCR Internalization Assays
GPCR internalization assays are functional, cell-based assays that measure GPCR endocytosis in live cells without a microscope, imaging or antibodies.

Products

Internalization Assays

Pharmacotrafficking Assays

Translocation Assays