Kinase Product Solutions

Kinase Enzymes and Cell-based Functional, Binding, and Activity Assays for Discovery through Development and for QC Lot-release Testing

Kinases play a central in cell signaling to elicit many physiological responses. Dysregulation of kinase activity leads to many human diseases, especially cancer. A typical signaling pathway involves a kinase cascade to produce a cellular response starting with receptor activation via a ligand-induced activation step. Receptor-specific ligands, such as growth factors, result in receptor tyrosine kinases (RTK) dimerization or oligomerization. This often results in the receptors’ activation and autophosphorylation, allowing them to recruit and activate downstream mediators via their SH2 domains. This event then triggers a signaling cascade that leads to the phosphorylation and activation of downstream kinases to act upon various downstream target molecules, including several cytoplasmic targets and transcriptional factors. The activated targets elicit cellular responses that include the promotion of cell growth and division, protein translation, or other physiological responses.

Eurofins DiscoverX® offers an extensive portfolio of functional and binding cell-based assays, cell lines, ready-to-use kits (including enzyme activity assay kits and bioassays), and recombinant kinase enzymes to evaluate any kinase target-based signaling activities to aid your efforts from discovery through development into commercialization. Screen for novel kinase inhibitors, perform pharmacological profiling, and evaluate kinase activation from therapeutic binding. Utilize cell-based assays to determine therapeutic mechanism of action (MOA) and intracellular compound target engagement. Perform kinase activity assays measuring ADP production or substrate phosphorylation with one of the most extensive offerings of recombinant kinase enzymes available.

As the new home of the original Millipore (Upstate) kinase enzyme portfolio covering over >70% of the kinome combined with the clinically-proven PathHunter® MOA-reflective, cell-based assay platform, Eurofins DiscoverX has the kinase solutions you need to integrate into your discovery and development pipelines to accelerate your drug discovery programs.

Product Highlights
  • Comprehensive Portfolio – Binding and functional, cell-based assays for receptor dimerization, SH2 recruitment, and target engagement as well as over 420 kinases active, mutant, and inactive recombinant kinases for activity and biophysical testing
  • Highly Applicable – Ideal for the development of small molecule and biologic therapeutics, from target ID through pre-clinical drug development programs, into therapeutic commercial release
  • Clinically Relevant, Cell-Based Assays – Leverage and implement any of the DiscoverX qualified or target-based bioassays to accelerate your biological candidate, whether it is a novel therapeutic or a biosimilar
  • Rigorously Tested Kinase Enzymes – Kinase enzymes utilized in KinaseProfiler services for screening millions of compounds over the past 30 years

Consider Eurofins DiscoverX’s custom development capabilities for custom cell lines, assays, & enzyme development.

Kinase Activity Assay Kits
Kinase Activity Assay Kits
Validated, ready-to-use enzyme activity assays to assess small molecule enzyme & kinase inhibitors in drug discovery
ADP Accumulation Assays
ADP Accumulation Assays
ADP accumulation assays detect kinase activity & inhibition by measuring ADP accumulation in antibody-free and homogeneous assay formats.
Receptor Tyrosine Kinase Assays
Receptor Tyrosine Kinase Assays
Cell-based functional assays to assess dimerization, phosphorylation, internalization, & SH2-recruitment of RTK & cytokine receptors associated with C...Show More

Products

Product Types

  • Recombinant Kinase and Phosphatase Enzymes - Over 420 high-quality active, inactive, unactive, and mutant kinases, including many exclusive enzymes, including the PI3K and the PIKK group (ATM, ATR, mTOR, and DNA-PK). Learn more about Recombinant Proteins.
  • ADP Accumulation Assays - Antibody-free, homogeneous, and gain-of-signal activity-based assays for identifying and characterizing kinase phosphotransferase activity. To learn more about ADP Accumulation Assays.
  • Kinase Enzyme Activity Assay Kits - Validated, ready-to-use assays allow for identifying and characterizing kinase enzyme activity and inhibition. To learn more about Enzyme Activity Assay Kits.
  • Kinase Receptor Assays - Functional and activity assays to analyze RTK dimerization, phosphorylation, internalization, and SH2-recruitment of RTKs. To learn more about RTK Assays.
  • Kinase Bioassay Kits - Qualified and target-based bioassays for evaluating RTK and cytokine receptor dimerization, SH2-recruitment, and IκB degradation, including CSF2, EGF, FGF, GH, IGF1, Insulin Receptor, Interleukin, ErbB2 (via HER2), PDGF, TrkA/B/C, and VEGF receptors including KDR. Learn more about Bioassay Kits.
  • Kinase eXpress Assay Kits - Ready-to-use cell-based assay kits for quick analysis and implementation in early discovery phases for screening applications, proof-of-concept, mechanism of action confirmation, and rank-ordering studies. Learn more about eXpress Assay Kits.
  • Kinase Cell Lines - Stable cell lines developed to reflect the target’s mechanism of action with a drug interaction in a standard cell line background. Learn more about Stable Cell Lines.

Applications