Signaling Pathway Product Solutions

Interrogate a Variety of Popular Target-Specific Pathways with Cell-Based Assays

Cellular pathway signaling constitutes key mechanisms that transfer biological information within cells for intracellular responses crucial in many physiological processes, including cell proliferation, differentiation, metabolic control, and apoptosis. Cell signaling pathways are activated when ligands (e.g., cytokines, growth factors, or hormones) bind to specific receptors on the cell surface. Detecting specific stimuli via intracellular signal pathways becomes paramount in interrogating the mechanism of action of the target ligand/receptor interaction for advancing drug discovery and development in many pathophysiologic and pharmacologic mechanisms.

Eurofins DiscoverX® offers a comprehensive collection of cell-based pathway indicator assays designed to detect activation or inhibition of complex signal transduction pathways in response to a small molecule or biologic. The assays are based on the established PathHunter® technology, and feature indicator cell lines used in pathway assays that enable you to measure distinct events within a variety of pathways associated with immune response, compound toxicity, cholesterol metabolism, antioxidant function, DNA damage, and more.

Combined with a fast and simple chemiluminescent detection, these signaling pathway assays enable the generation of cellular pathway selectivity profiles and obtain a comprehensive understanding of different compounds or biologics for receptor-proximal or -distal events without relying on complex phenotypic screens.

Product Highlights
  • Homogeneous Assays – Simple, no wash HTS-friendly protocols with a sensitive chemiluminescent read-out
  • Multiple Assay Types – Binding and functional (direct and reporter-based) cell-based assay with no need for antibodies, fixing, or imaging
  • Fast, Superior Results – Rapid assay with short compound incubation times, high signal-to-background, and no false positives
  • High Specificity – Whole cell, non-ELISA assays with increased target specificity

Consider Eurofins DiscoverX’s custom development capabilities for custom cell lines, assays, & enzyme development.

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